The workflow main goal is to run divers tools to obtain alignment quality metrics from RNA-Seq, DNA-Seq, and ChIP-Seq using Picard, Qualimap, Samtools, dupradar, biotype analysis, FastQ Screen, RSeQC or Phantom peak. Finally it runs Multi QC to gather tool metrics. The workflow was designed to be run in the SeqUIa (http://cfb.ceitec.muni.cz/sequia) application.