**Workflow for Metagenomics binning from assembly.
**
Minimal inputs are: Identifier, assembly (fasta) and an associated sorted BAM file
Summary
- MetaBAT2 (binning)
- MaxBin2 (binning)
- SemiBin2 (binning)
- Binette (bin merging)
- EukRep (eukaryotic classification)
- CheckM2 (bin completeness and contamination)
- BUSCO (bin completeness)
- GTDB-Tk (bin taxonomic classification)
- CoverM (bin abundances)
Including:
**Bin annotation (workflow: https://workflowhub.eu/workflows/1170):**
- Bakta
- Interproscan
- Eggnog
- KOfamscan
- To RDF conversion with SAPP (optional, default on) --> https://workflowhub.eu/workflows/1174/
Other UNLOCK workflows on WorkflowHub: https://workflowhub.eu/projects/16/workflows?view=default
**All tool CWL files and other workflows can be found here:**
https://gitlab.com/m-unlock/cwl
**How to setup and use an UNLOCK workflow:**
https://docs.m-unlock.nl/docs/workflows/setup.html