demux_doublet_sim

Repository for Nextflow pipeline used in demuxSNP demultipelxing paper

Overall workflow

1. Simulate doublets

• Add per-sample suffix to barcodes in BAM
• Merge per-sample BAMs
• Generate lookup of barcodes to rename to reach a set % doublets
• Rename barcodes in BAM as per lookup

2. Benchmark methods

• Experiments 1: Vary doublet rate
• Experiment 2: Vary SNP subsetting

Inputs

Most inputs are specified in nextflow.config: container__souporcell: path to souporcell apptainer ...

PaSTa is a nextflow-based end-to-end image analysis pipeline for decoding image-based spatial transcriptomics data. It performs imaging cycle registration, cell segmentation and transcripts peak decoding. It is currently supports analysis of three types of ST technology:

• in-situ sequencing-like encoding
• MERFISH-like encoding
• RNAScope-like labelling

Prerequisites:

1. Nextflow. Installation guide: https://www.nextflow.io/docs/latest/getstarted.html
2. Docker or Singularity. Installation guide: ...

A workflow for performing alignment and phylogeny using protein sequences when studying genes/gene families.

EC-Earth3 workflow without wrappers running in MeluXina with Autosubmit v3.15.14, used to assess the effects of task aggregation on queueing times. Workflow configuration is based on the Auto-EC-Earth3's testing suite [1].

In order to reduce the size of the workflow, the /tmp directory has been deleted. Additionally, the experiment has been cleaned up with the Autosubmit clean command. The ...

EC-Earth3 workflow without wrappers running in MareNostrum 5 with Autosubmit v3.15.18, used to assess the effects of task aggregation on queueing times. Workflow configuration is based on the Auto-EC-Earth3's testing suite [1].

In order to reduce the size of the workflow, the /tmp directory has been deleted. Additionally, the experiment has been cleaned up with the Autosubmit clean command. ...

EC-Earth3 workflow with wrappers running in MareNostrum 5 with Autosubmit v3.15.18, used to assess the effects of task aggregation on queueing times. Workflow configuration is based on the Auto-EC-Earth3's testing suite [1].

In order to reduce the size of the workflow, the /tmp directory has been deleted. Additionally, the experiment has been cleaned up with the Autosubmit clean command. The ...

EC-Earth3 workflow without wrappers running in MareNostrum 4 with Autosubmit v3.15.0b0, used to assess the effects of task aggregation on queueing times. Workflow configuration is based on the Auto-EC-Earth3's testing suite [1].

In order to reduce the size of the workflow, the /tmp directory has been deleted. Additionally, the experiment has been cleaned up with the Autosubmit clean command. ...

EC-Earth3 workflow with wrappers running in MareNostrum 4 with Autosubmit v3.15.0b0, used to assess the effects of task aggregation on queueing times. Workflow configuration is based on the Auto-EC-Earth3's testing suite [1].

In order to reduce the size of the workflow, the /tmp directory has been deleted. Additionally, the experiment has been cleaned up with the Autosubmit clean command. ...

RNA-SeqEZPZ-NF

Nextflow Pipeline for RNA-SeqEZPZ

A Point-and-Click Pipeline for Comprehensive Transcriptomics Analysis with Interactive Visualizations

RNA-SeqEZPZ-NF is another implementation of RNA-SeqEZPZ. RNA-SeqEZPZ-NF uses the same user interface as RNA-SeqEZPZ and runs the same pipeline, but runs the pipeline implemented by Nextflow. This pipeline is currently tested on HPC cluster with SLURM scheduler. Advanced ...

RNA-SeqEZPZ: A Point-and-Click Pipeline for Comprehensive Transcriptomics Analysis with Interactive Visualizations

RNA-SeqEZPZ is a pipeline to run analysis of RNA-Seq experiments from raw FASTQ files all the way to differential genes analysis. The pipeline is accessible through a graphical user interface implemented using a Shiny app and features interactive plots. Advanced users have the ability to customize the scripts provided with the pipeline. This pipeline is designed to run on an HPC ...