Workflows
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Type: Common Workflow Language
Creators: Ryo Mameda, Sora Yonezawa
Submitters: Sora Yonezawa, Ryo Mameda
Purge contigs marked as duplicates by purge_dups (could be haplotypic duplication or overlap duplication). The contigs are purged from the first assembly (hap1, pri...), added to the second assembly (hp2, alt... ), then the 2nd assembly is purged as well. If you think only one of the assemblies needs purging, use the VGP6b workflow. This workflow is the 6th workflow of the VGP pipeline. It is meant to be run after one of the contigging steps (Workflow 3, 4, or 5).
Generate a genome assembly based on PacBio HiFi reads. Part of the VGP suite, it needs to be run after the VGP1 k-mer profiling workflow. The assembly contigs are built using HiFiasm, and the workflow generates assembly statistics, BUSCO reports, Merqury plots, and the contigs in fasta and GFA formats.
Generate phased assembly based on PacBio HiFi reads and parental Illumina data for phasing. Part of the VGP workflow suite, it needs to be run after the Trio k-mer Profiling workflow VGP2. This workflow uses HiFiasm for contigging, and generates assembly statistics, BUSCO reports, Merqury plots, and the genome assembly contigs in fasta and GFA format.
Purge contigs marked as duplicates by purge_dups in a single haplotype (could be haplotypic duplication or overlap duplication). If you think the purged contigs might belong to the other haplotype, use the workflow VGP6 instead. This workflow is the 6th workflow of the VGP pipeline. It is meant to be run after one of the contigging steps (Workflow 3, 4, or 5).
pb_variants
A snakemake 9 based Pipeline for hifi snp, sv, cnv calling, phasing and more
Only PacBio data for now
!!THIS PIPLINE IS IN-DEVELOPMENT AND EXPERIMENTAL, USE AT YOUR OWN RISK!!
what this tools aims to deliver:
- newest and best tools suited for HiFi data (only for now)
- singletons and trio analysis (trio is coming sometime...)
- human-first (hg38 for now), others should be possible (untested...)
included tools:
- deepvariant or bcftools for snp calling
- snps get used for ...
Text mining a museum collection in tabular format to extract from which year most objects derive and what they are.
Associated Tutorial
This workflows is part of the tutorial OpenRefine Tutorial for researching cultural data, available in the GTN
Features
- Includes [Galaxy Workflow ...
MAGNETO is an automated snakemake workflow dedicated to MAG (Metagenome-Assembled Genomes) reconstruction from metagenomic data.
It includes a fully-automated coassembly step informed by optimal clustering of metagenomic distances, and implements complementary genome binning strategies, for improving MAG recovery.
Key Features
- Quality Control (QC): Automatically assesses the quality and the contamination of input reads, ensuring that low-quality data are filtered out to improve downstream ...
Type: Snakemake
Creators: Samuel Chaffron, Audrey Bihouee, Benjamin Churcheward, Maxime Millet, Guillaume Fertin, Hugo Lefeuvre
Submitter: Hugo Lefeuvre
This project is an analysis pipeline using Snakemake for RNAseq analysis in order to find differentially expressed genes. It has been widely tested on human RNA sequencing from an Illumina HiSeq but should work on most systems and many other species, provided the necessary resource files can be downloaded.
Description
This pipeline is set for paired-end data only from Illumina HiSeq output files.
The main steps of the pipeline are:
- optionnal cleaning data with ...