Workflows
What is a Workflow?Filters
This project is an analysis pipeline using Snakemake for RNAseq analysis in order to find differentially expressed genes. It has been widely tested on human RNA sequencing from an Illumina HiSeq but should work on most systems and many other species, provided the necessary resource files can be downloaded.
Description
This pipeline is set for paired-end data only from Illumina HiSeq output files.
The main steps of the pipeline are:
- optionnal cleaning data with ...
This workflow performs the scaffolding of a genome assembly using HiC data with YAHS. Can be used on any assembly with Hi-C data, and the assembly in the gfa format.
To perform Qualification, Nitrate Calibration, Validation of One Argo float or One Glider by using Neural Network and Climatology. Copy past before running and change calibration parametrization if necessary
Associated Tutorial
This workflows is part of the tutorial Nitrate DMQC for autonomous platforms such as Argo floats, available in the GTN ...
Soil Metagenome Pipeline
Soil Metagenome Pipeline is a modular, Nextflow DSL2 workflow for assembling, polishing, binning, annotating, and functionally characterizing complex soil metagenomes. It orchestrates state-of-the-art tools for long- and short-read metagenomics, generates high-quality MAGs, assigns taxonomy, and screens for biosynthetic gene clusters (BGCs).
What it does
- Assembles long-read metagenomes (e.g., ONT) with Flye and optionally polishes with Medaka and/or NextPolish using ...
The workflow main goal is to align DNA-Seq read files with BWA-MEM , with optional adapter trimming, UMI consensus calling, duplicate marking. Finally it runs Multi QC to gather tool metrics. The workflow was designed to be run in the SeqUIa (http://cfb.ceitec.muni.cz/sequia) application.
The workflow main goal is to run divers tools to obtain alignment quality metrics from RNA-Seq, DNA-Seq, and ChIP-Seq using Picard, Qualimap, Samtools, dupradar, biotype analysis, FastQ Screen, RSeQC or Phantom peak. Finally it runs Multi QC to gather tool metrics. The workflow was designed to be run in the SeqUIa (http://cfb.ceitec.muni.cz/sequia) application.
The workflow main goal is to count reads aligned or pseudo-aligned to reference genome annotation using featureCount, HTSeq-count, RSEM, Kallisto or Salmon. Finally it runs Multi QC to gather tool metrics. The workflow was designed to be run in the SeqUIa (http://cfb.ceitec.muni.cz/sequia) application.
The workflow main goal is to align RNA-Seq read files with STAR and to check for duplicates using either Picard Markduplicates or UMI-tools. Finally it runs Multi QC to gather tool metrics. The workflow was designed to be run in the SeqUIa (http://cfb.ceitec.muni.cz/sequia) application.
The workflow serves as a short introduction to Galaxy for users from the Humanities who mostly work with texts. The workflow compares two texts, visualises the differences with 'diff' and a wordcloud and extracts selected passaged for further analysis.
Associated Tutorial
This workflows is part of the tutorial Introduction to Digital Humanities in Galaxy, available in ...