Hi-C Analysis with Galaxy Tutorial Workflow
1.0

Workflow Type: Galaxy

Workflow for the GTN tutorial: "Hi-C analysis of Drosophila melanogaster cells using HiCExplorer".

Associated Tutorial

This workflows is part of the tutorial Hi-C analysis of Drosophila melanogaster cells using HiCExplorer, available in the GTN

Features

Thanks to...

Workflow Author(s): Tristan Reynolds

Tutorial Author(s): Joachim Wolff, Fidel Ramirez, Vivek Bhardwaj, Ekaterina Polkh

Tutorial Contributor(s): Tristan Reynolds, Helena Rasche, Saskia Hiltemann, Nicola Soranzo, Joachim Wolff, Björn Grüning, Mehmet Tekman, Anup Kumar, Torsten Houwaart, Bérénice Batut, Niall Beard

Funder(s): ELIXIR Europe, de.NBI, University of Freiburg, Collaborative Research Centre 992, The University of Melbourne, Melbourne Bioinformatics, Australian BioCommons

gtn star logo followed by the word workflows

Inputs

ID Name Description Type
GM12878_25kb_cooler_coarsen.cool GM12878_25kb_cooler_coarsen.cool n/a
  • File
H3K27me3.bw H3K27me3.bw n/a
  • File
H3K36me3.bw H3K36me3.bw n/a
  • File
H4K16ac.bw H4K16ac.bw n/a
  • File
HiC_S2_1p_10min_lowU_R1.fastq.gz HiC_S2_1p_10min_lowU_R1.fastq.gz n/a
  • File
HiC_S2_1p_10min_lowU_R2.fastq.gz HiC_S2_1p_10min_lowU_R2.fastq.gz n/a
  • File
corrected_contact_matrix_dm3_large.h5 corrected_contact_matrix_dm3_large.h5 n/a
  • File
dm3_genes.bed dm3_genes.bed n/a
  • File
dm3_genome.fasta dm3_genome.fasta n/a
  • File

Steps

ID Name Description
9 Bowtie2 toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.3+galaxy1
10 Bowtie2 toolshed.g2.bx.psu.edu/repos/devteam/bowtie2/bowtie2/2.5.3+galaxy1
11 hicFindRestSite toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicfindrestrictionsites/hicexplorer_hicfindrestrictionsites/3.7.6+galaxy1
12 hicInfo toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicinfo/hicexplorer_hicinfo/3.7.6+galaxy1
13 hicDetectLoops toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicdetectloops/hicexplorer_hicdetectloops/3.7.6+galaxy1
14 hicInfo toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicinfo/hicexplorer_hicinfo/3.7.6+galaxy1
15 hicPCA toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicpca/hicexplorer_hicpca/3.6+galaxy1
16 hicFindTADs toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicfindtads/hicexplorer_hicfindtads/3.7.6+galaxy1
17 hicBuildMatrix toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicbuildmatrix/hicexplorer_hicbuildmatrix/3.7.6+galaxy1
18 hicPlotMatrix toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicplotmatrix/hicexplorer_hicplotmatrix/3.7.6+galaxy1
19 hicPlotMatrix toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicplotmatrix/hicexplorer_hicplotmatrix/3.7.6+galaxy1
20 hicPlotMatrix toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicplotmatrix/hicexplorer_hicplotmatrix/3.7.6+galaxy1
21 pyGenomeTracks toolshed.g2.bx.psu.edu/repos/iuc/pygenometracks/pygenomeTracks/3.8+galaxy2
22 hicCorrectMatrix toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hiccorrectmatrix/hicexplorer_hiccorrectmatrix/3.7.6+galaxy1
23 hicMergeMatrixBins toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicmergematrixbins/hicexplorer_hicmergematrixbins/3.7.6+galaxy1
24 hicCorrectMatrix toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hiccorrectmatrix/hicexplorer_hiccorrectmatrix/3.7.6+galaxy1
25 hicPlotMatrix toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicplotmatrix/hicexplorer_hicplotmatrix/3.7.6+galaxy1
26 hicPlotMatrix toolshed.g2.bx.psu.edu/repos/bgruening/hicexplorer_hicplotmatrix/hicexplorer_hicplotmatrix/3.7.6+galaxy1

Version History

1.0 (earliest) Created 11th Aug 2025 at 14:14 by GTN Bot

Added/updated 4 files


Open master b504113
help Creators and Submitter
Creators
Not specified
Submitter
Discussion Channel
Activity

Views: 33   Downloads: 3   Runs: 0

Created: 11th Aug 2025 at 14:14

help Attributions

None

Total size: 222 KB
Powered by
(v.1.17.0-main)
Copyright © 2008 - 2025 The University of Manchester and HITS gGmbH