Portable genotype-free demultiplexing benchmarkign pipeline.

A portable pipeline for benchmarking genotype-free single-cell demultiplexing methods on simulated data.

The pipeline is designed to be generelisable to different datasets with arbitrary numbers of simulated mulitplexed samples. All software as part of pipeline is run through Apptainer containers to ensure reproducibility and ease of use. The pipeline default configuration is to be run on a cluster with a SLURM scheduler, but can be ...

demux_doublet_sim

Repository for Nextflow pipeline used in demuxSNP demultipelxing paper

Overall workflow

1. Simulate doublets

• Add per-sample suffix to barcodes in BAM
• Merge per-sample BAMs
• Generate lookup of barcodes to rename to reach a set % doublets
• Rename barcodes in BAM as per lookup

2. Benchmark methods

• Experiments 1: Vary doublet rate
• Experiment 2: Vary SNP subsetting

Inputs

Most inputs are specified in nextflow.config: container__souporcell: path to souporcell apptainer ...

PaSTa is a nextflow-based end-to-end image analysis pipeline for decoding image-based spatial transcriptomics data. It performs imaging cycle registration, cell segmentation and transcripts peak decoding. It is currently supports analysis of three types of ST technology:

• in-situ sequencing-like encoding
• MERFISH-like encoding
• RNAScope-like labelling

Prerequisites:

1. Nextflow. Installation guide: https://www.nextflow.io/docs/latest/getstarted.html
2. Docker or Singularity. Installation guide: ...

A workflow for performing alignment and phylogeny using protein sequences when studying genes/gene families.

EC-Earth3 workflow with wrappers running in MeluXina with Autosubmit v3.15.14, used to assess the effects of task aggregation on queueing times. Workflow configuration is based on the Auto-EC-Earth3's testing suite [1].

In order to reduce the size of the workflow, the /tmp directory has been deleted. Additionally, the experiment has been cleaned up with the Autosubmit clean command. The ...

EC-Earth3 workflow without wrappers running in MeluXina with Autosubmit v3.15.14, used to assess the effects of task aggregation on queueing times. Workflow configuration is based on the Auto-EC-Earth3's testing suite [1].

In order to reduce the size of the workflow, the /tmp directory has been deleted. Additionally, the experiment has been cleaned up with the Autosubmit clean command. The ...

EC-Earth3 workflow without wrappers running in MareNostrum 5 with Autosubmit v3.15.18, used to assess the effects of task aggregation on queueing times. Workflow configuration is based on the Auto-EC-Earth3's testing suite [1].

In order to reduce the size of the workflow, the /tmp directory has been deleted. Additionally, the experiment has been cleaned up with the Autosubmit clean command. ...

EC-Earth3 workflow with wrappers running in MareNostrum 5 with Autosubmit v3.15.18, used to assess the effects of task aggregation on queueing times. Workflow configuration is based on the Auto-EC-Earth3's testing suite [1].

In order to reduce the size of the workflow, the /tmp directory has been deleted. Additionally, the experiment has been cleaned up with the Autosubmit clean command. The ...

EC-Earth3 workflow without wrappers running in MareNostrum 4 with Autosubmit v3.15.0b0, used to assess the effects of task aggregation on queueing times. Workflow configuration is based on the Auto-EC-Earth3's testing suite [1].

In order to reduce the size of the workflow, the /tmp directory has been deleted. Additionally, the experiment has been cleaned up with the Autosubmit clean command. ...

EC-Earth3 workflow with wrappers running in MareNostrum 4 with Autosubmit v3.15.0b0, used to assess the effects of task aggregation on queueing times. Workflow configuration is based on the Auto-EC-Earth3's testing suite [1].

In order to reduce the size of the workflow, the /tmp directory has been deleted. Additionally, the experiment has been cleaned up with the Autosubmit clean command. ...