Workflows
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demux_doublet_sim
Repository for Nextflow pipeline used in demuxSNP demultipelxing paper
Overall workflow
- Simulate doublets
- Add per-sample suffix to barcodes in BAM
- Merge per-sample BAMs
- Generate lookup of barcodes to rename to reach a set % doublets
- Rename barcodes in BAM as per lookup
- Benchmark methods
- Experiments 1: Vary doublet rate
- Experiment 2: Vary SNP subsetting
Inputs
Most inputs are specified in nextflow.config: container__souporcell: path to souporcell apptainer ...
Type: Nextflow
Creators: Michael Lynch, Leverages scripts developed by Weber et al (2021) DOI: https://doi.org/10.1093/gigascience/giab062
Submitter: Michael Lynch
PaSTa is a nextflow-based end-to-end image analysis pipeline for decoding image-based spatial transcriptomics data. It performs imaging cycle registration, cell segmentation and transcripts peak decoding. It is currently supports analysis of three types of ST technology:
- in-situ sequencing-like encoding
- MERFISH-like encoding
- RNAScope-like labelling
Prerequisites:
- Nextflow. Installation guide: https://www.nextflow.io/docs/latest/getstarted.html
- Docker or Singularity. Installation guide: ...
YOLO based tail analysis workflow for image segmentation and detection. The workflow is based on https://git.embl.org/grp-cba/tail-analysis/-/blob/main/analysis_workflow.md
Associated Tutorial
This workflows is part of the tutorial Training Custom YOLO Models for Object Detection and Segmentation in Bioimages, available in the GTN
...
From Obis data to Biodiversity indicators
Associated Tutorial
This workflows is part of the tutorial Obis marine indicators, available in the GTN
Features
- Includes Galaxy Workflow Tests
Thanks to...
Workflow Author(s): Marie Jossé
Tutorial Author(s): ...
Secondary metabolite biosynthetic gene cluster (SMBGC) Annotation using Neural Networks Trained on Interpro Signatures
Associated Tutorial
This workflows is part of the tutorial Marine Omics identifying biosynthetic gene clusters, available in the GTN
Features
- Includes Galaxy Workflow Tests ...
Associated Tutorial
This workflows is part of the tutorial From NDVI data with OpenEO to time series visualisation with Holoviews, available in the GTN
Features
- Includes Galaxy Workflow Tests
Thanks to...
Workflow Author(s): Marie Jossé
**Tutorial ...
This workflow allows you to annotate a genome with Helixer and evaluate the quality of the annotation using BUSCO and Genome Annotation statistics. GFFRead is also used to predict protein sequences derived from this annotation, and BUSCO and OMArk are used to assess proteome quality.
Associated Tutorial
This workflows is part of the tutorial Genome annotation with Helixer, available ...
Refining Genome Annotations with Apollo
Associated Tutorial
This workflows is part of the tutorial Refining Genome Annotations with Apollo (prokaryotes), available in the GTN
Thanks to...
Tutorial Author(s): Anthony Bretaudeau, [Helena ...
Type: Galaxy
Creators: Anthony Bretaudeau, Helena Rasche, Nathan Dunn, Mateo Boudet
Submitter: GTN Bot
Masking repeats in a genome using RepeatMasker
Associated Tutorial
This workflows is part of the tutorial Masking repeats with RepeatMasker, available in the GTN
Features
- Includes Galaxy Workflow Tests
Thanks to...
Workflow Author(s): Anthony ...
Type: Galaxy
Creators: Anthony Bretaudeau, Alexandre Cormier, Laura Leroi, Erwan Corre, Stéphanie Robin, Jonathan Kreplak
Submitters: GTN Bot, Armin Dadras